Generator

Part:BBa_K743015

Designed by: Bernardo Pollak   Group: iGEM12_UC_Chile   (2012-09-22)

after dusk -> LuxAB luciferase (Vibrio fischerii)

This part is designed for the expression of the LuxA and LuxB genes from Vibrio fischeri in a circadian manner with peak 2 hours after subjective dusk (or hour 14 of circadian time).

When transfromed into Synechocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.

This part allows the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 693
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 521
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2080


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