Part:BBa_K743008
psb1C3_IntKR recombination plasmid for Synechocystis PCC6803
This plasmid is intended to be used as a starting backbone for further addition of DNA parts by Gibson assembly between RS1 and B0015. As Synechocystis PCC6803 undergoes double homologous recombination naturally, any sequence between RS1 and RS2 will be introduced into its chromosome along with a KanR gene for selection in cyanobacteria or in E. coli.
The plasmid backbone also has a Chloramphenicol resistance casette for selection in E. coli only.
The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells.
This part was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis PCC6803.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1758
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1758
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1758
Illegal XhoI site found at 2255 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1758
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1758
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511
Verification by Colony PCR
psb1C3_IntK plasmid with BBa_K743008 digested with EcoR1 and Pst1
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