Composite
psb1C3_int

Part:BBa_K743006

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)

psb1C3_IntK recombination plasmid for Synechocystis PCC6803

This plasmid is intended to be used as a starting backbone for further addition of DNA parts by Gibson assembly between RS1 and KanR. As Synechocystis PCC6803 undergoes double homologous recombination naturally , any sequence between RS1 and RS2 will be introduced into its chromosome along with a KanR gene for selection in cyanobacteria or in E. coli.

The plasmid backbone also has a Chloramphenicol resistance casette for selection in E.coli only. The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells.

This part was used by UC_Chile 2012 team to succesfully transform Synechocystis PCC6803.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1774
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1774
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1774
    Illegal XhoI site found at 2271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1774
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


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Categories
Parameters
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