Generator

Part:BBa_K741009

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)

pCon(0.856)-RBS-crogfp-T

Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 124
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918

USTC2012Result5 副本.png

Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).

Data

                                         pCon(0.856)-RBS-crogfp-T
　                   OD                   FLU
t/min Parallel Experimental group M9 medium Parallel Experimental group M9 medium
0 1 0.068 1 611.19
2 0.0696 2 560.005
3 0.0741 0.0416 3 540.949 14.037
45 1 0.0787 1 625.166
2 0.0757 2 587.647
3 0.0746 0.0402 3 617.612 14.013
90 1 0.0858 1 712.785
2 0.0827 2 673.465
3 0.0814 0.0411 3 648.529 14.449
135 1 0.0939 1 769.154
2 0.0887 2 740.309
3 0.0877 0.0419 3 747.658 13.826
180 1 0.1018 1 800.945
2 0.0913 2 801.508
3 0.0953 0.0396 3 862.744 14.123
225 1 0.1182 1 903.339
2 0.0989 2 936.183
3 0.1128 0.0474 3 912.456 14.706
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Categories
Parameters
None