Generator

Part:BBa_K741008

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)

pCon(0.244)-RBS-crogfp-T

Here, pCon(J23114) constantly expressing the fusion protein crogfp(BBa_K741006) at 10% of the strength of pCon(J23100).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 124
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918

USTC2012Result5 副本.png

Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).

Data

                                            pCon(0.244)-RBS-crogfp-T
　 OD fluorescence intensity
t/min Parallel  Experimental group M9 medium Parallel  Experimental group M9 medium
0 1 0.136 0.0559 1 132.355 18.095
　 2 0.1313 　 2 116.709 　
45 1 0.1988 0.0522 1 180.537 19.878
　 2 0.2004 　 2 172.497 　
90 1 0.3153 0.0543 1 267.399 21.225
　 2 0.3126 　 2 243.579 　
135 1 0.445 0.0541 1 346.814 20.907
　 2 0.4478 　 2 357.696 　
180 1 0.4949 0.0533 1 423.673 21.545
　 2 0.5014 　 2 454.136 　
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Categories
Parameters
None