Part:BBa_K737068

GFP generator

We improved the existing partBBa_I20260

The absolute activity of BioBrick promoters varies across experimental conditions and measurement instruments. Researchers should choose one promoter (BBa_J23101) to serve as an in vivo reference standard for promoter activity. Drew Endy, etc demonstrated that, by measuring the activity of promoters relative to BBa_J23101, we could reduce variation in reported promoter activity due to differences in test conditions and measurement instruments by ~50%. They defined a Relative Promoter Unit (RPU) in order to report promoter characterization data in compatible units and developed a measurement kit so that researchers might more easily adopt RPU as a standard unit for reporting promoter activity. [1] However, unfortunately, there are few data about it and few teams use it as an in vivo control. This year, we detected many RFU (Relative Fluorescence Unit) curves and made it as control group. Our team wants to upload some data about it and hope other teams can use it to decrease the error.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 706