Composite

Part:BBa_K737062

Designed by: Wenjie Wu and Minghao Gong   Group: iGEM12_OUC-China   (2012-09-19)

GalK_Constitutive anti-messenger RNA generator

This device mean to construct IPTG/aTc NOR GATE. AntiGalK stands for the RNA transcripted fully complementary to galK,which shows modest repression on galk::GFP mRNA. Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.

Spot42 sRNA is under the control of aTc inducible device,another RNA antiGalK is under the control of IPTG inducible device.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aGalK_Constitutive anti-messenger RNA generator