Part:BBa_K737056
YtfJ_s1s2_Gnt
The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit. Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.
Spot42 sRNA is under control of aTc inducible device,the buffer RNA YtfJ is under control of IPTG inducible device.IPTG/aTc concentration gradient results in different YtfJ RNA/sRNA Spot42 levels in cells, which can be monitored by our galK::GFP generator(where galK is the RBS of GFP and the target for Spot42) ,Spot42 can be buffered by YtfJ_Spot42 Scaffold fusion RNA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1231
Illegal NheI site found at 1254 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2432
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1181
- 1000COMPATIBLE WITH RFC[1000]
| n/a | YtfJ_s1s2_Gnt |