Part:BBa_K737055

NanC_s1s2_Gnt

The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit. Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one. Spot42 sRNA is under control of aTc inducible device,the buffer RNA NanC is under control of IPTG inducible device.IPTG/aTc concentration gradient results in different NanC RNA/sRNA Spot42 levels in cells, which can be monitored by our galK::GFP generator(where galK is the RBS of GFP and the target for Spot42) ,Spot42 can be buffered by NanC_Spot42 Scaffold fusion RNA.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 1439
  Illegal NheI site found at 1462
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2640
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1261
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1228