Composite

Part:BBa_K737052

Designed by: Weihong Lai and Peiran Zhang   Group: iGEM12_OUC-China   (2012-09-19)

aTc inducible GFP Generaror

[J23106,P0440]is a constitutive TetR repressor generator,fluorescence can be observed with induced by aTc. The RBS of E0240 is B0030,a weak GFP translational unit specialized to quantitively describe the behaviour of promotor in many cases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1616


Experimental Data=

K737052.jpg

In the picture we can see the good inductive efficiency of aTc.

Note

R0040 don’t work well in high copy number plasmids.

[edit]
Categories
Parameters
n/aaTc inducible GFP Generaror