Composite

Part:BBa_K737051

Designed by: Weihong Lai and Peiran Zhang   Group: iGEM12_OUC-China   (2012-09-19)

IPTG inducible GFP Generator

[J23106,P0412]is a constitutive lacI repressor generator,Fluorescence can be observed with induced by IPTG. The RBS of E0240 is B0030,a weak GFP translational unit specialized to quantitively describe the behaviour of promotor in many cases.

9-1.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2085


Note

R0011 doesn’t work well in high copy number plasmids.

[edit]
Categories
Parameters
n/aIPTG inducible GFP Generator