Composite

Part:BBa_K737047

Designed by: Wenjie Wu and Minghao Gong   Group: iGEM12_OUC-China   (2012-09-19)

GalK_Comparator

This device mean to test feasibility of N/P NOR GATE,replacing Nitrate,Phosphate inputs by IPTG,aTc. Spot42 sRNA is under the control of aTc inducible device,another RNA antiGalK is under the control of IPTG inducible device. AntiGalK stands for the RNA transcripted fully complementary to galK,shows modest repression on galK::GFP mRNA. Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.

9-1.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1096
    Illegal NheI site found at 1119
    Illegal NheI site found at 2634
    Illegal NheI site found at 2657
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3446


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