Part:BBa_K737046

SrlA_Comparator

The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit. This device mean to test feasibility of N/P comparator,replacing Nitrate,Phosphate inputs by IPTG,aTc Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.

Spot42 sRNA is under control of aTc inducible device,the buffer RNA SrlA is under control of IPTG inducible device.

The pairing hybridization energy is -12.2 kcal/mol. 14 bases are involved in the pairing. Microarray analysis indicates that it could give as strong as more than 10-fold repression rate when srlA expression is activatively induced

Experimental Data

Figure 1 ODE simulation of comparator

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 1375
  Illegal NheI site found at 1398
  Illegal NheI site found at 2913
  Illegal NheI site found at 2936
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2576
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3725