Composite

Part:BBa_K737030

Designed by: Minghao Gong   Group: iGEM12_OUC-China   (2012-09-18)

orthogonal ratio sensor[tNanC::spot42(mut)_Gnt


This is the altered RNA element of AACGU which introduced corresponding 5 bases mutant in NanC leading sequence for complementary pairing with ACGUU. It is one of the two elements for construction of orthogonal RNA-mediated ratio sensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None