Part:BBa_K737018
J23106+B0034+gvpC-20psi+B0015
This part conteins a constitutive promoter J23106, a strong RBS B0034, a coding sequence gvpC-20psi and a double-terminator B0015. This part was examined by SDS-PAGE. We transform this part to Escherichia coli Top10 strain. After culturing in Luria-Bertani broth (with Chloramphenicol) for 24 hours, the E. coli cells was centrifuged at the speed of 8000 rpm. Then we resuspent the cell in 50ul PBS (0.5mol/L) and add 50ul 2XLoading buffer in the EP tube. After boiling in burning water for 5min, the cells was centrifuged at 8000 rpm. Then add 20ul supernate to sample hole and SDS-Paged it at 80V for spacer gel and 120V for separation gel. Finally, we got the gel image and analysed it. At last, we located the protein at 20kDa, which means the protein gvpA was successfully expressed in E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |