Composite

Part:BBa_K736003

Designed by: Lethbridge iGEM High School Team   Group: iGEM12_Lethbridge_Canada   (2012-06-16)


pLAC-regulated TAT-RFP construct

This construct tests for the ability of the TAT sequence to cause secretion of protein outside of the cell, using RFP as a reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 923


[edit]
Categories
Parameters
None