Reporter

Part:BBa_K736001

Designed by: Lethbridge iGEM High School Team   Group: iGEM12_Lethbridge_Canada   (2012-06-16)


TAT signal sequence fused to red fluorescent protein (E1010)

The twin argenine tag (TAT) sequence targets proteins to the membrane for secretion. This signal sequence contains the conserved T-R-R-X-F-L-K sequence motif at the end terminus.

The red fluorescent protein is used as a proof of principle to confirm that the TAT signal sequence is capable of sequestering the protein outside of the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 706
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 695


[edit]
Categories
Parameters
None