Reporter
Part:BBa_K736001
Designed by: Lethbridge iGEM High School Team Group: iGEM12_Lethbridge_Canada (2012-06-16)
TAT signal sequence fused to red fluorescent protein (E1010)
The twin argenine tag (TAT) sequence targets proteins to the membrane for secretion. This signal sequence contains the conserved T-R-R-X-F-L-K sequence motif at the end terminus.
The red fluorescent protein is used as a proof of principle to confirm that the TAT signal sequence is capable of sequestering the protein outside of the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 706
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 695
[edit]
Categories
Parameters
None |