Regulatory

Part:BBa_K736000

Designed by: Lethbridge iGEM High School Team   Group: iGEM12_Lethbridge_Canada   (2012-06-16)


Constitutive Promoter (J23100) with MLC (glucose-regulated) binding site

This part is designed to regulate transcription of downstream genes according to glucose concentration.

In the absence of glucose in Escherichia coli cells, MLC is bound to DNA; this prevents transcription. When glucose enters the cell, a phosphoryl group from an endogenous protein (EIIBC-gluc) is transferred to the glucose. When EIIBC-gluc is unphosphorylated, it recruits the MLC protein; that is, the MLC becomes unbound from the DNA, which allows transcription to occur.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None