Plasmid

Part:BBa_K734006

Designed by: Ben Slater   Group: iGEM12_Austin_Texas   (2012-10-01)

pReporter.permaflipped control for ZombiE.coli

This control plasmid is the “permaflipped” version of BBa_K734005 (pReporter for ZombiE.coli), whose sequence is identical to that of the reporter plasmid but has the promoter flipped, as if already acted upon by CRE-Recombinase. Using this control will show whether Z-Mode ("infected") cells actually express the RFP when CRE-Recombinase is assumed to work as intended, flipping the promoter region and generating one double-mutant and one wild-type loxP site. When transformed into cells and induced with IPTG, all cells should glow red if this control works correctly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2353
    Illegal PstI site found at 3300
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2353
    Illegal PstI site found at 3300
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2353
    Illegal PstI site found at 3300
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2353
    Illegal PstI site found at 3300
    Illegal NgoMIV site found at 5477
    Illegal NgoMIV site found at 5637
    Illegal AgeI site found at 568
    Illegal AgeI site found at 846
    Illegal AgeI site found at 958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3474
    Illegal BsaI.rc site found at 70
    Illegal SapI.rc site found at 4556


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