Composite

Part:BBa_K734004

Designed by: Razan Alnahhas   Group: iGEM12_Austin_Texas   (2012-09-25)


sfGFP regulated by LasR quorum sensing pathway

sfGFP coding region under control of LasR promoter and LasR coding region under control of LacUV5.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5486
    Illegal PstI site found at 3199
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2412
    Illegal NheI site found at 4644
    Illegal PstI site found at 3199
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5486
    Illegal PstI site found at 3199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5486
    Illegal PstI site found at 3199
    Illegal NgoMIV site found at 1898
    Illegal AgeI site found at 1701
    Illegal AgeI site found at 3462
    Illegal AgeI site found at 4730
    Illegal AgeI site found at 5054
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3367


[edit]
Categories
Parameters
None