Composite

Part:BBa_K734003

Designed by: Razan Alnahhas   Group: iGEM12_Austin_Texas   (2012-09-25)


CRE regulated by LasR quorum sensing pathway

The plasmid contains the coding region of CRE recombinase under the LasR promoter and the LasR coding region under the Lacuv5 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3566
    Illegal XbaI site found at 6100
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3566
    Illegal NheI site found at 2412
    Illegal NheI site found at 5258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3566
    Illegal BamHI site found at 2878
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3566
    Illegal XbaI site found at 6100
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3566
    Illegal XbaI site found at 6100
    Illegal NgoMIV site found at 1898
    Illegal AgeI site found at 1701
    Illegal AgeI site found at 2564
    Illegal AgeI site found at 4076
    Illegal AgeI site found at 5344
    Illegal AgeI site found at 5668
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None