Composite

Part:BBa_K732002

Designed by: Joshua Fass   Group: iGEM12_Virginia   (2012-10-01)

synCG-β + Promoter + RBS

Human chorionic gonadotropin (hCG) is a hormone produced during pregnancy. Its beta subunit (hCG-β) contains epitopes recognized by pregnancy tests. The full hCG dimer is also used as an ovulation inducer and can stimulate the production of testosterone in men.

This sequence could ideally be used to express hCG for any downstream uses, and also as an output signal since it could trigger pregnancy tests at low concentrations when properly folded.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 224
    Illegal NgoMIV site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prc
//function/reporter
Parameters
None