Coding

Part:BBa_K730002

Designed by: Antresh Kumar   Group: iGEM12_JUIT-India   (2012-09-26)


The complete denitrification of nitrate by bacteria to dinitrogen (N,)

The complete denitrification of nitrate by bacteria to dinitrogen (N,) is generally an anaerobic respiratory process. The last step involves the dissimilatory reduction of nitrous oxide (N,O), the free energy change of which can be coupled to phosphorylation (Zumft,1992; Zumft & Kroneck, 1990). nosZ is the structural gene for the periplasmic N,O reductase which is required to the conversion of nitrous oxide into free nitrogen. . The nitrous oxide that is emitted in the paddy fields is caused due to the excessive use of fertilizers. The microbes naturally present in the paddy field lead to the emission of nitrous oxide by utilizing these chemical fertilizers. . NosZ is a gene derived from P.aeruginosa and is used for denitrification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1845
    Illegal NgoMIV site found at 908
    Illegal NgoMIV site found at 1072
    Illegal AgeI site found at 282
    Illegal AgeI site found at 1381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1638
    Illegal BsaI.rc site found at 667
    Illegal BsaI.rc site found at 1312


[edit]
Categories
Parameters
n/aThe complete denitrification of nitrate by bacteria to dinitrogen (N,)