Part:BBa_K729016

Antifreeze protein, type I from Oceanibulbus indolifex HEL-45,

Antifreeze protein from Oceanibulbus indolifex

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Description

This is a public biobrick. The true creators of this biobrick are the citizen scientists at the London Hackspace.The initial stage of cloning was conducted at the London Hackspace based in Hoxton, and transformation into E. coli W3110 cells took place at UCL laboratory to comply with UK/EU regulations.

An account of the workshops, during which the BioBrick was assembled, is available on the [http://2012.igem.org/Team:University_College_London/HumanPractice/DIYbio/Workshops UCL iGEM 2012] wiki. A detailed lab book is available on the [http://wiki.london.hackspace.org.uk/view/Public_Biobrick London Hackspace Wiki].

List of Contributors

Nicholas Nickleby: Primer design & DIY incubator software

William Beaufoy: Biobrick construction

Simon Rose:Biobrick construction

Tonderai Ra:Biobrick construction

Paddy Smith:Biobrick construction & DIY incubator/shaker circuit

Ji-Won Kim:Biobrick construction