Composite

Part:BBa_K729000

Designed by: Bouran Sohrabi   Group: iGEM12_University_College_London   (2012-06-25)


Temperature regulated Gas Vesicle Polycistronic gene cluster composite

This composite consists of the gas vesicle polycistronic gene regulated by heat shock promoter BBa_K338001, repressor dapA and corresponding promoter dapAp, such that a reduction in temperature induces greater expression of the gvp gene cluster.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 188
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1594
    Illegal BamHI site found at 5399
    Illegal XhoI site found at 5087
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2045
    Illegal NgoMIV site found at 2447
    Illegal AgeI site found at 5366
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3857
    Illegal SapI.rc site found at 5013


[edit]
Categories
Parameters
None