Composite

Part:BBa_K727727

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)


FMO3 Generator

FMO3 (flavin monooxygenase 3) is an enzyme that catalyses the oxidation of TMA (trimethylamine) into TMAO (trimethylamine oxide) which results as the removal of unpleasant fish odor. The promoter is attached into the part with the aim of easier regulation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2669
    Illegal PstI site found at 2222
    Illegal PstI site found at 2585
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1053
    Illegal SpeI site found at 2670
    Illegal PstI site found at 2222
    Illegal PstI site found at 2585
    Illegal PstI site found at 2684
    Illegal NotI site found at 1059
    Illegal NotI site found at 2677
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1053
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2670
    Illegal PstI site found at 2222
    Illegal PstI site found at 2585
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1053
    Illegal XbaI site found at 1068
    Illegal SpeI site found at 2670
    Illegal PstI site found at 2222
    Illegal PstI site found at 2585
    Illegal PstI site found at 2684
    Illegal NgoMIV site found at 1936
    Illegal AgeI site found at 1436
    Illegal AgeI site found at 1915
    Illegal AgeI site found at 2057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


[edit]
Categories
Parameters
None