Plasmid

Part:BBa_K727700

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)


pSB1K3 with RFP coding device

We added RFP coding region into the basic pSB1K3 plasmid backbone with aim of using it during our trasnformations. RFP will indicate the false ligations.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3252
    Illegal suffix found in sequence at 1070
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3252
    Illegal SpeI site found at 1071
    Illegal PstI site found at 1085
    Illegal NotI site found at 1078
    Illegal NotI site found at 3258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3252
    Illegal XhoI site found at 2102
    Illegal XhoI site found at 3128
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3252
    Illegal suffix found in sequence at 1071
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3252
    Illegal XbaI site found at 3267
    Illegal SpeI site found at 1071
    Illegal PstI site found at 1085
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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