Composite

Part:BBa_K727272

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)


Fish Odor Generator-Complete System

TMT (trimethylamine transferase) takes part in the third reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a negative feedback response with the help of producing LuxR and HSL and activating the promoter with them.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 160
    Illegal suffix found in sequence at 1554
    Illegal EcoRI site found at 1583
    Illegal EcoRI site found at 3033
    Illegal XbaI site found at 1598
    Illegal XbaI site found at 3048
    Illegal SpeI site found at 3005
    Illegal SpeI site found at 4536
    Illegal PstI site found at 3019
    Illegal PstI site found at 4550
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 160
    Illegal EcoRI site found at 1583
    Illegal EcoRI site found at 3033
    Illegal SpeI site found at 1555
    Illegal SpeI site found at 3005
    Illegal SpeI site found at 4536
    Illegal PstI site found at 1569
    Illegal PstI site found at 3019
    Illegal PstI site found at 4550
    Illegal NotI site found at 166
    Illegal NotI site found at 1562
    Illegal NotI site found at 1589
    Illegal NotI site found at 3012
    Illegal NotI site found at 3039
    Illegal NotI site found at 4543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 160
    Illegal EcoRI site found at 1583
    Illegal EcoRI site found at 3033
    Illegal BglII site found at 1535
    Illegal BglII site found at 1707
    Illegal BglII site found at 4402
    Illegal BglII site found at 5985
    Illegal BamHI site found at 3166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 160
    Illegal suffix found in sequence at 1555
    Illegal EcoRI site found at 1583
    Illegal EcoRI site found at 3033
    Illegal XbaI site found at 1598
    Illegal XbaI site found at 3048
    Illegal SpeI site found at 3005
    Illegal SpeI site found at 4536
    Illegal PstI site found at 3019
    Illegal PstI site found at 4550
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 160
    Illegal EcoRI site found at 1583
    Illegal EcoRI site found at 3033
    Illegal XbaI site found at 175
    Illegal XbaI site found at 1598
    Illegal XbaI site found at 3048
    Illegal SpeI site found at 1555
    Illegal SpeI site found at 3005
    Illegal SpeI site found at 4536
    Illegal PstI site found at 1569
    Illegal PstI site found at 3019
    Illegal PstI site found at 4550
    Illegal NgoMIV site found at 4111
    Illegal AgeI site found at 498
    Illegal AgeI site found at 573
    Illegal AgeI site found at 1314
    Illegal AgeI site found at 1968
    Illegal AgeI site found at 1977
    Illegal AgeI site found at 2728
    Illegal AgeI site found at 3383
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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