Composite

Part:BBa_K727011

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)


Fish Odor Generator-Component Three

MMT (monomethylamine transferase) catalyses the first reaction of TMA (trimethylamine) synthesis. TMA is the major cause of unpleasant fish odor.DMT (dimethylamine transferase) takes part in the second reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor.TMT (trimethylamine transferase) takes part in the third reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor. The promoter is attached into the part with the aim of easier regulation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2447
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 3926
    Illegal XbaI site found at 2491
    Illegal XbaI site found at 3941
    Illegal SpeI site found at 3898
    Illegal SpeI site found at 5429
    Illegal PstI site found at 3912
    Illegal PstI site found at 5443
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1053
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 3926
    Illegal SpeI site found at 2448
    Illegal SpeI site found at 3898
    Illegal SpeI site found at 5429
    Illegal PstI site found at 2462
    Illegal PstI site found at 3912
    Illegal PstI site found at 5443
    Illegal NotI site found at 1059
    Illegal NotI site found at 2455
    Illegal NotI site found at 2482
    Illegal NotI site found at 3905
    Illegal NotI site found at 3932
    Illegal NotI site found at 5436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1053
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 3926
    Illegal BglII site found at 2428
    Illegal BglII site found at 2600
    Illegal BglII site found at 5295
    Illegal BamHI site found at 4059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1053
    Illegal suffix found in sequence at 2448
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 3926
    Illegal XbaI site found at 2491
    Illegal XbaI site found at 3941
    Illegal SpeI site found at 3898
    Illegal SpeI site found at 5429
    Illegal PstI site found at 3912
    Illegal PstI site found at 5443
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1053
    Illegal EcoRI site found at 2476
    Illegal EcoRI site found at 3926
    Illegal XbaI site found at 1068
    Illegal XbaI site found at 2491
    Illegal XbaI site found at 3941
    Illegal SpeI site found at 2448
    Illegal SpeI site found at 3898
    Illegal SpeI site found at 5429
    Illegal PstI site found at 2462
    Illegal PstI site found at 3912
    Illegal PstI site found at 5443
    Illegal NgoMIV site found at 5004
    Illegal AgeI site found at 1391
    Illegal AgeI site found at 1466
    Illegal AgeI site found at 2207
    Illegal AgeI site found at 2861
    Illegal AgeI site found at 2870
    Illegal AgeI site found at 3621
    Illegal AgeI site found at 4276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


[edit]
Categories
Parameters
n/aFish Odor Generator-Component Three