Coding

Part:BBa_K726011

Designed by: Veronica Zepeda   Group: iGEM12_UCSF   (2012-10-02)


T7Prom+HisTag+YefM

This part contains YefM the antitoxin of the yefM-yoeB toxin-antitoxin system. It is behind a T7 Promoter with a , lac operator, ribosome binding site, and 6x his tag. The construct can be moved to any plasmid for expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None