Signalling

Part:BBa_K726007

Designed by: Sami Chu   Group: iGEM12_UCSF   (2012-08-10)


T7 driven lac operated inducer for the las quorum-sensing system

Produces chemical system PAI-1. Induces the promoter pLasR with the lasR regulatory protein. Requires T7 RNA polymerase and inducable by lactose.

Part of a two-strain self-inducing system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 796
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 358
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None