Regulatory

Part:BBa_K726003

Designed by: Sami Chu   Group: iGEM12_UCSF   (2012-08-09)

T7 promoter + lac operator + His tag T7 promoter is good for protein expression in e. coli. Requires the T7 RNA polymerase. Also contains a lac operator (inducable with lactose/ lactose substitute), and a His tag for protein purification. Insert your protein of interest behind this.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None