Coding

Part:BBa_K726002

Designed by: Sami Chu   Group: iGEM12_UCSF   (2012-08-08)

LasR Regulated Melanin-Producing Construct

The first part is the LasR promoter [[1]], and the second part is the gene that codes for melanin [[2]]. By placing the LasR regulator in front of this composite part, it will produce melanin when induced with PAI-1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1377
    Illegal BsaI.rc site found at 682


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Categories
Parameters
None