Other

Part:BBa_K678022

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)


Neomycin cassette

Resistance cassette for selection of mammalian cells. The cassette contains a SV40 promoter, the neomycin resistance gene, and the SV40 poly A as a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 591
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 591
    Illegal NgoMIV site found at 1042
    Illegal NgoMIV site found at 1325
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 891
    Illegal SapI.rc site found at 1101


[edit]
Categories
//cds/selectionmarker/antibioticresistance
//chassis/eukaryote/human
//direction/forward
Parameters
None