Device

Part:BBa_K672001

Designed by: Emmanuel Garuz   Group: iGEM11_UTP-Panama   (2011-09-28)

New Promoter For Periplasmic Heat Generation

This part was designed with the idea of improving the expression of AOX1A in response to a cold shock. To do that we combined the CsPA Promoter + Cold Box + UTR + SD + DB (Part:BBa_K328001) with the OmpA + AOX1A previusly ensambled by iGEM10_GeorgiaTech.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 322
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None