Coding

Part:BBa_K669005

Designed by: Silvia J Cañas   Group: iGEM11_Colombia   (2011-09-28)


CBP + promoter

Chitooligosaccharide-Binding Protein (CBP) is a cytoplasmatic protein that regulates the transcriptional activity of the ChiS sensor.

When chitin is present in the external media, CBP binds with GlcNAc oligomers (chitin degradation products) and liberates the sensor for the up-regulation of the chitinolitic degradation cascade in Vibrio fischeri ES114.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 276
    Illegal SpeI site found at 573
    Illegal PstI site found at 119
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 276
    Illegal SpeI site found at 573
    Illegal PstI site found at 119
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 276
    Illegal SpeI site found at 573
    Illegal PstI site found at 119
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 276
    Illegal SpeI site found at 573
    Illegal PstI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None