Device

Part:BBa_K663002

Designed by: Alexander Twaddle   Group: iGEM11_SouthBend-Mishawaka-HS   (2011-06-23)


Wintergreen and GFP-generators on an homoserine lactone responsive promoter

F2620, encodes a transcription factor, LuxR (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a TetR (BBa_C0040) regulated operator (BBa_R0040) has coupled to both the GFP-generator (E0840) and the wintergreen smell/BSMT-1-generator (J45119) to form a device that glows green in ultraviolet light and smells like wintergreen when HSL from bacteria is present in water.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3247
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1734
    Illegal BsaI.rc site found at 2959


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Categories
Parameters
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