Composite

Part:BBa_K663000

Designed by: Alexander Twaddle   Group: iGEM11_SouthBend-Mishawaka-HS   (2011-06-20)


Homoserine lactone sensor

The Green Fluorence Protein Reporter BBa_E0840,is coupled to BBa_F2620,A composite part comosed of a transcription factor, LuxR (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a TetR (BBa_C0040) regulated operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as aTc can be used to produce a logical AND function.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1734


[edit]
Categories
Parameters
None