Regulatory

Part:BBa_K660616

Designed by: Ellin-Kristina Hillert   Group: iGEM11_Glasgow   (2011-09-22)


pBAD with strong RBS

This regulatory part is a composite of a strong pBAD promoter (Part:BBa_K206000) and a strong ribosome binding site (Part:BBa_B0034), for use in selective expression of any device. Using this composite will simplify the making of constructs, as it reduces the number of ligations necessary to produce a full pBAD controlled construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None