Composite

Part:BBa_K660603

Designed by: Glasgow Uni 2011   Group: iGEM11_GlasgowUni   (2011-09-21)

OmpF + W.RBS

This is a composite part featuring the OmpF promoter and Weak RBS.

It is part of the 2011 University of Glasgow Promoter/RBS library.

Design

The small size of the ribosome binding site means that it cannot be visualised on a gel. To solve this problem, we used AlwnI restriction enzyme in a method that is detailed on our wiki.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None