Coding

Part:BBa_K660300

Designed by: Glasgow Uni 2011   Group: iGEM11_GlasgowUni   (2011-09-16)


Phosphodiesterase (enzyme)

Phosphodiesterases are a family of enzymes naturally present in microorganisms, which break phosphodiester bonds. The specific enzyme we are using breaks the phosphodiester bond in the second messenger nucleotide, cyclic digaunylate (cyclic-di-GMP). Cyclic-di-GMP is important in many bacterial processes, including biofillm formation and motility. The enzyme regulates signal transduction by controlling levels of the signalling molecule in cells.

We are using the enzyme to control the levels of cyclic-di-GMP within the cell. We expect that the targeted expression of phosphodesiterase could be used to interfere with biofilm formation or to trigger dispersal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 140
    Illegal PstI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 140
    Illegal PstI site found at 316
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 140
    Illegal PstI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 140
    Illegal PstI site found at 316
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 257
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 672
    Illegal NgoMIV site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 220


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Parameters
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