Part:BBa_K658004

a bacteria population-control device with RBS0.3 driven by lacl+pL

The device is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri.It is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.3 was used to regulate the expression of killer protein ccdB.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 718
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2345
  Illegal BsaI.rc site found at 1874