Part:BBa_K658002
a bacteria population-control device with RBS0.6 driven by lacl+pL
The device is a twin part of Biobrick part BBa_K658001. This device is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.6 was used to regulate the expression of killer protein ccdB.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 876
Illegal NheI site found at 899 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
Illegal BamHI site found at 1513
Illegal XhoI site found at 2655 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1840
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5061
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