Regulatory

Part:BBa_K656010

Designed by: Evan Clark, Julius Ho, Eli Moss, Jesse Palmer   Group: iGEM11_Brown-Stanford   (2011-09-19)

Anabaena pSac Promoter

Anabaena vegetative cell promoter, linked to photosystem I. Can be used to express specifically in vegetative cells, excluding heterocyst expression.

This promoter sequence is derived from a region upstream of the pSac operon in Anabaena 7120. The operon belongs to photosystem I; therefore the promoter's activity is effectively constitutive in that organism, in growth conditions. However, Anabaena has the important characteristic of cell differentiation under nitrogen deficient conditions, forming non-photosynthetic cells called heterocysts. The more common vegetative cell type will express genes under control of the pSac promoter, but the heterocyst cells will not.

We transformed Anabaena 7120 with a construct containing GFP reporter controlled by psaC promoter part BBa_K656010. Bright field light micrograph and GFP filter images are compared to demonstrate fluorescence of a successful transformant. Red arrows point to heterocyst cells containing noticeably less fluorescence.

Transformed Anabaena carrying a GFP reporter controlled by psaC promoter part BBa_K656010, Bright field image


Transformed Anabaena carrying a GFP reporter controlled by psaC promoter part BBa_K656010, GFP filter; Red arrows point to heterocysts


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None