Plasmid
Part:BBa_K654095
Designed by: Cody Tramp Group: iGEM11_Utah_State (2011-09-27)
RFP Cloning Control pSB1A3_IntC6803
This is the base pSB1A3_IntC6803 Synechocystis sp. PCC 6803 cloning integration plasmid, with an RFP expression device in the BioBrick site. This makes cloning constructs into the plasmid easier, as the RFP is a negative control. Simply digest this plasmid and your desired construct with EcoRI and PstI, and then ligate the construct into the plasmid backbone. Any non-recombinant plasmids will appear visibly red/pink on the plates, allowing for easy selection of successful transformants.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 6741
Illegal suffix found in sequence at 770 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6741
Illegal NheI site found at 2335
Illegal SpeI site found at 771
Illegal PstI site found at 785
Illegal NotI site found at 778
Illegal NotI site found at 6747 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6741
Illegal BglII site found at 2133
Illegal BamHI site found at 4097
Illegal XhoI site found at 6614 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 6741
Illegal suffix found in sequence at 771 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 6741
Illegal XbaI site found at 6756
Illegal SpeI site found at 771
Illegal PstI site found at 785
Illegal NgoMIV site found at 1468
Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1583
Illegal BsaI.rc site found at 3280
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