Plasmid

Part:BBa_K654095

Designed by: Cody Tramp   Group: iGEM11_Utah_State   (2011-09-27)


RFP Cloning Control pSB1A3_IntC6803

This is the base pSB1A3_IntC6803 Synechocystis sp. PCC 6803 cloning integration plasmid, with an RFP expression device in the BioBrick site. This makes cloning constructs into the plasmid easier, as the RFP is a negative control. Simply digest this plasmid and your desired construct with EcoRI and PstI, and then ligate the construct into the plasmid backbone. Any non-recombinant plasmids will appear visibly red/pink on the plates, allowing for easy selection of successful transformants.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6741
    Illegal suffix found in sequence at 770
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6741
    Illegal NheI site found at 2335
    Illegal SpeI site found at 771
    Illegal PstI site found at 785
    Illegal NotI site found at 778
    Illegal NotI site found at 6747
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6741
    Illegal BglII site found at 2133
    Illegal BamHI site found at 4097
    Illegal XhoI site found at 6614
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6741
    Illegal suffix found in sequence at 771
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6741
    Illegal XbaI site found at 6756
    Illegal SpeI site found at 771
    Illegal PstI site found at 785
    Illegal NgoMIV site found at 1468
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1583
    Illegal BsaI.rc site found at 3280


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