Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
Device

Part:BBa_K651000

Designed by: Sung won Lim, Hannah Landes, Balvir Kunar   Group: iGEM11_NYC_Software   (2011-09-26)


RecA promoter with RFP coding device

This is a demo wetlab project performed by the 2011 NYC iGEM Software team to introduce them to basic practices of molecular biology, and to explore the steps and tools involved in putting together a simple genetic device.

E.Coli recA promoter which had been colony PCR'd out of JM109 strain with RFC23 overhangs was combined with a ribosome binding site and mRFP1 E1010 coding device.

This device is designed to produce RFP in presence of any external stimuli that can trigger the recA promoter. For purposes of NYC iGEM Software and our sister team NYC iGEM Wetware we focused on the device's ability to act as an ionizing radiation sensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 747
    Illegal AgeI site found at 859
    Illegal AgeI site found at 1695
    Illegal AgeI site found at 2910
    Illegal AgeI site found at 3022
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None