We characterized BBa_K649104 and BBa_K117002 in E.coli lsrR(-).
We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a gfp gene downstream of the promoter. Its fluorescence intensity was much higher than that of promoterless-gfp(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of lsrA promoter-gfp((BBa_K117002)-gfp) was almost the same as a promoterless-gfp(negative control), showing that lsrA promoter(BBa_K117002) does not work properly.
We improved previous lsrA promoter(BBa_K117002).our assay of BBa_K117002
For more information, see our work in Tokyo_Tech 2011 wiki.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 770