Composite

Part:BBa_K648132

Designed by: Lauren Rossi   Group: iGEM11_Penn_State   (2011-10-28)


Pr of Or tester with Cro

This is a test circuit comprised of mCherry, operating region (Or) of lambda phage, pBAD, and Cro with RBS B0034. When the circuit is induced, Cro is expressed. Cro will bind to the binding sites of the operating region.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1101
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1195
    Illegal BamHI site found at 1041
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPr of Or tester with Cro