Composite

Part:BBa_K648130

Designed by: Lauren Rossi   Group: iGEM11_Penn_State   (2011-10-28)


Pr of Or tester with cI repressor with very weak RBS

This is a test circuit comprised of mCherry, operating region (Or) of lambda phage, pBAD, and the cI repressor with RBS B0031. When the circuit is induced, the cI repressor is expressed. cI repressor will bind to the binding sites of the operating region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1101
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1041
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a Pr of Or tester with cI repressor with very weak RBS