Composite

Part:BBa_K648129

Designed by: Lauren Rossi   Group: iGEM11_Penn_State   (2011-10-28)


Pr of Or tester with cI repressor with slightly weak RBS

This is a test circuit comprised of mCherry, operating region (Or) of lambda phage, pBAD, and the cI repressor with RBS B0030. When the circuit is induced, the cI repressor is expressed. cI repressor will bind to the binding sites of the operating region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1101
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1041
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPr of Or tester with cI repressor with slightly weak RBS